My Coop Experience

Humira Sample Bag for Daily analysis  

Manufacturing Operators collect the sample every day and delivered to Technical Operation Department for several tests.

Vi-CELL Series Cell Viability Analyzer

  All manufacturing samples and Humira special project samples are measured for cell viability, cell density and cell diameter.   The Vi-CELL used the trypan blue dye exclusion method for cell analysis.

Centrifuge 

The centrifuge is used every day to precipitate the cells and obtain the product secreted in the media. 

Cedex Bio Analyzer

The Cedex Bio is an automated analyzer used for the determination of lactate, glucose, glutamine and Immunoglobulin G (IgG) concentrations. These measurements are performed after the sample is centrifuge. 

Storage 

After the analysis of the samples are collected in micro-tubes and bottles, the samples are then stored at -80 Celsius degrees.

AKTA Chromatography System

This systems is used for protein purification after few days of the samples storing. 

CHO cell adaptation and Scale-up Process 

The lab scale-bioreactors projects initiate with the CHO cell adaptation in shakers or glass spinners in order to increase the amount of cells for the cell inoculation. 

I-Control Bioreactors 

These scale-bioreactors of 3 liters are used for the fermentation process of special projects with the objective to optimize the upstream process of Humira production. 

Syringe used for Project Daily Sampling 

Syringe of different volumes are used to collected the sample from the bioreactors. 

ABL 5 Gas Analyzer

The ABL 5 gas analyzer is used to measured the values of pH, carbon dioxide and dissolved oxygen in the samples. 

Osmometer 

The osmometer analyzes the osmotic strength of the media and cell solution of the bioreactors. 

GraviTrap Columns Kit

This columns kit is used for protein purification process for the samples obtained after the harvest* of the bioreactors. 

*Harvest process is when we centrifuge the samples and separate the cells from the supernatant.